Journal: Journal of Animal Science

Article Title: Effects of increasing Fe dosage in newborn pigs on suckling and subsequent nursery performance and hematological and immunological criteria

doi: 10.1093/jas/skaa221

Figure Lengend Snippet: Effects of Fe dosage on immune function criteria at weaning (21-d postfarrowing) 1

Article Snippet: TNF-α response was measured by subjecting blood samples to 800 μL of lipopolysaccharide (LPS) and placing in 96-well enzyme-linked immunosorbent assay (ELISA) plates coated with anti-swine TNF-α polyconal antibody (Kingfisher, Biotech, Inc., St. Paul, MN) and absorbance was measured using a microtech spectrometer (BioTek EON, Winooski, VT).

Techniques: Clinical Proteomics

Journal: Frontiers in Veterinary Science

Article Title: Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response

doi: 10.3389/fvets.2020.00009

Figure Lengend Snippet: Interleukin (IL)-10 (A) , IL-12 (B) , IL-8 (C) , interferon-(IFN)-γ, (D) , tumor necrosis factor (TNF)-α, and (E) secretion by PBMCs unstimulated (UNC) and stimulated with LPS and ConA (SC, 1 and 5 μg/ml, respectively) and with 4 mM of H 2 O 2 for 24 h at 37°C/5% CO 2 . * P < 0.05 were considered significant among feeding strategy.

Article Snippet: Biotinylated secondary anti-bovine IFN-γ antibody (2 μg/ml, final concentration, AbD Serotec) and anti-bovine TNF-α antibody (2.5 μg/ml, final concentration in PBS, Kingfisher Biotech) dissolved in PBS were added for 45 min. For the IL-8 antibody conjunction, the rabbit anti-sheep interleukin-8 (2 μg/ml, final concentration in PBS, AbD Serotec) was used and added to the wells for 1 h. After washing four times, 100 μl of streptavidin–horseradish peroxidase (HRP) (1/500, AbD Serotec) was added for IFN-γ and TNF-α assay, whereas, for IL-8, a goat anti-rabbit IgG HRP-conjugated antibody (1/20,000 in PBS, Sigma-Aldrich, Italy) was added into wells.

Techniques:

Journal: Immunity, Inflammation and Disease

Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells

doi: 10.1002/iid3.93

Figure Lengend Snippet: Reduced anti‐viral cytokine production in BLV‐infected cattle. IFN‐γ (A) and TNF‐α (B) in the supernatants of cultured PBMCs from BLV‐infected aleukemic (AL: n = 9) or persistent lymphocytotic (PL: n = 7) cattle and uninfected cattle ( n = 4) were determined using ELISA. Detection of IFN‐γ producing CD4 + T cells in BLV‐uninfected and BLV‐infected cattle at different disease stages (C: BLV(−): n = 7, AL: n = 14, PL: n = 13). Correlations between IFN‐γ producing CD4 + T cells and lymphocyte numbers (D) and proviral loads (E), TNF‐α producing CD4 + T cells, lymphocyte numbers (F), and proviral loads (G) in BLV‐infected cattle ( n = 27). Lymphocyte numbers in BLV‐infected cattle were counted using Celltac α MEK‐6450 and animals were diagnosed with PL when at least two consecutive lymphocyte counts were >7,500/µL; proviral loads were quantified using real‐time PCR. IFN‐γ‐ and TNF‐α‐producing CD4 + T cells in BLV‐infected cattle were detected using flow cytometry.

Article Snippet: After 24 h, supernatants were harvested and IFN‐γ (Mabtech, Nacka Strand, Sweden) and TNF‐α (Kingfisher Biotech) production was determined using ELISA kits with a microplate reader MTP‐650FA (Corona Electric, Ibaraki, Japan) at an absorbance of 450 nm.

Techniques: Infection, Cell Culture, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Flow Cytometry

Journal: Immunity, Inflammation and Disease

Article Title: Bovine leukemia virus reduces anti‐viral cytokine activities and NK cytotoxicity by inducing TGF‐β secretion from regulatory T cells

doi: 10.1002/iid3.93

Figure Lengend Snippet: Inhibition of IFN‐γ and TNF‐α production from CD4 + T cells by TGF‐β. PBMCs from normal cattle (IFN‐γ: n = 12, TNF‐α: n = 9) and BLV‐infected cattle ( n = 11) were pretreated with TGF‐β for 2 h and were cultivated with IL‐2 alone (A and B) or synthesized peptides from the BLV envelop region (C and D). IFN‐γ‐ or TNF‐α‐producing CD4 + T cells were detected using flow cytometry.

Article Snippet: After 24 h, supernatants were harvested and IFN‐γ (Mabtech, Nacka Strand, Sweden) and TNF‐α (Kingfisher Biotech) production was determined using ELISA kits with a microplate reader MTP‐650FA (Corona Electric, Ibaraki, Japan) at an absorbance of 450 nm.

Techniques: Inhibition, Infection, Synthesized, Flow Cytometry